Effect of Aqua-Ethanolic Extract of Glaucium flavum on Diabetic Nephropathy Parameters in Rats

Abstract Background & aim: Glaucium flavum is used as an anti-diabetes herb in traditional medicine in the southern regions of Iran. Regarding the widespread secondary complications of diabetes, including nephropathy and hepatopathy. The present study was conducted to compare the effects of glacial auricularis and glibenclamide on the changes in serum levels of blood urea nitrogen, creatinine and total and direct bilirubin in alloxan-induced diabetic rats. Methods: In the present experimental study, 40 adult male Wistar rats were randomly divided into five equal groups: control, diabetic control, diabetic treatment with 250 mg / kg glaucium flavum extract, diabetic treated with mentioned extract with daily dose of 500 mg / kg and diabetic treatment with glibenclamide at daily dose of 5 μg / kg. Groups 2 to 5 received single dose (120 mg / kg) of alloxan. After thirty days, the animals were anesthetized and blood samples were taken from the heart. Creatinine, urea nitrogen, totals and direct bilirubin were measured by enzymatic method. The obtained cultivars were analyzed by one-way ANOVA and Tukey's post-hoc test. Results: A significant differences was seen between the means of 250 treated group and diabetic control group among all four factors (P<0.001). Moreover, the difference of means were not significant between 500 treated group and healthy control in BUN, creatinine, total and direct bilirubin (P>0.05), this reduction for the drug treated group was limited to creatinine and total bilirubin. Conclusion: It could be concluded that the oral administration of the aqueous extracts may help to improve the activity of liver and kidney cleansing of diabetic rats by using anti-inflammatory properties of its compounds, including glucosin, as well as the anti-oxidant effects of Berberine.